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Mechanism of glutathione transferase P1-1-catalyzed activation of the prodrug canfosfamide (TLK286, TELCYTA)

Antioxidants help prevent further damage after trauma or surgery by deactivating unstable free radicals

It recycles other antioxidants (vitamin C, glutathione) and chelates heavy metals

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Samples were digested overnight at 37 C in 50 l of 50 mM ammonium bicarbonate, shaking the plate at 1,650 rpm with trypsin and LysC at a 1:50 and 1:100 ratio, respectively