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doi: 10.1007/978-0-387-75681-3_30 66 MiyazakiTIshikuraKHondaARaSGKomineSMiyamotoYet al

Oct 1 2021;12(5):1911-1929

Pharmacopsychiatry 46 , 120121 (2012)

doi: 10.1177/0333102417702132 11

We give 1/3 of this dose over the first 2 h and 2/3 of this dose over the next 3 h

3.4 Enzymatic cleavage to remove GST affinity tag Depending on the vector chosen, the GST affinity tag can be removed with thrombin, factor Xa, or PreScission protease, either in solution or while still bound to the column matrix