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In the next, extremely unfavorable elimination step (step 2), the conjugate base of the amine is kicked out from the tetrahedral intermediate: The reaction is driven to completion in a quick, irreversible deprotonation of the carboxylic acid by the newly formed RNH

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IGF-1 LR3 can also be reconstituted with dilute acetic acid for long-term stability, but BAC water is common for immediate use

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