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Semaglutide was originally developed by Novo Nordisk as an antidiabetic agent under the brand name Ozempic (0.52 mg weekly, subcutaneous), approved by the FDA in 2017 for type 2 diabetes management

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Reduced glutathione is the biologically active form naturally utilized by the body and is the preferred form used in dietary supplements

Dietary factors also likely contribute to epigenetic modifications

Transfection was carried out using Lipofectamine 3000 Kit on cells at 50% confluence for 6 hours, followed by protein extraction 48 hours post-transfection

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