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Conflicts of Interest The authors declare no conflicts of interest

TschuckJTheilackerLRothenaignerIWeiSAIAkdoganBLamVTet al

Morphological methods like immunohistochemistry, in situ hybridization or PCR from liver specimens play no relevant role in the diagnosis of hepatitis C because of their low sensitivity, poor specificity and low efficacy compared to serologic and nucleic acid-based approaches

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Collect the cell lysate and centrifuge at 13,200 g at 4 C for 15 min, and the supernatants were used for LCMS analysis after diluting with water to proper concentrations

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