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Following the first reaction step, the At LDOX protein was removed by filtering the reaction through an Amicon Ultra-4 (10 kDa cut-off) (Merck Millipore) and distributing 90 l aliquots in 96-well microtitre plates (PS, F-Bottom, clear, Greiner)

In this study, articular chondrocytes were expanded to a number that was needed for ACI