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In the negative control, normal rabbit IgG (ab172730, Abcam, Cambridge, UK) diluted to 4 g/mL in 1% rabbit serum in PBS was used instead of the primary antibodies

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Interestingly, body mass over 2 weeks was shown to remain constant across 11 different ambient temperatures, between 5 and 35 C, in both male and female mice 6

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A case series of four patients in Aesthetic Surgery Journal Open Forum reported that facial measurements held steady or improved when a hyperdiluted calcium hydroxylapatite biostimulator was used during active weight loss, with patients losing an average of 24.3 pounds over the study period

While these represent uncontrolled single-person reports rather than systematic evidence, they align with the preclinical research findings