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Also addition of solution of glutathione (GSH) transferase in the reaction mixture caused a marked increase in the rate of reaction compared to the non-enzymatic, showing monobromobimane was a substrate for this enzyme ( Figure 1 )

There is a ~ 2-hour delay in the onset of its effect because insulin is injected as a complex of insulin & protamine, and insulin is not free to become absorbed until protamine is digested by proteolytic enzymes present in body tissues

Using a larger vial with more water keeps a sizeable dose under the 1 mL mark