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A total of 3.0 ml reaction mixture contained 50 l of sample, 2.640 ml of 0.1 M phosphate buffer (pH,6.5), 0.3 ml of 1 mM reduced glutathione and 10 l of 1 mM 1 chloro-2,4 dinitrobenzene (CDNB)
Swallowed absorption
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As an essential amino acid, it cannot be synthesized de novo by the human body due to a lack of the require metabolic pathway needed for its synthesis
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