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Ki67 staining The immunohistochemistry of Ki67 was conducted using mouse anti-Ki67 antibodies (Thermo Fisher, Shanghai, PA5-114437) and HRP-labeled goat anti-mouse antibodies (Servicebio, Wuhan, G23301) along with DAB chromogenic agent kit for histochemistry (Servicebio, Wuhan, GB12224)

-arrestins were first recognized for desensitizing GPCRs by uncoupling them from G-proteins and promoting receptor internalization via clathrin-coated pits, but are now known to act as signaling scaffolds themselves, initiating distinct pathways like ERK, JNK, or NF-B activation

1. Appetite Reduction and Satiety Enhancement

Negishi, Y., Nobusada, K

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