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You have to ultimately consider the pros and cons of your situation

10.1016/j.phymed.2018.11.020 Summary Keywords acetaminophen, liver injury, natural products, oxidative stress, Nrf2 Citation Liao J, Lu Q, Li Z, Li J, Zhao Q and Li J (2023) Acetaminophen-induced liver injury: Molecular mechanism and treatments from natural products

Additionally, short study durations and standardized laboratory conditions restrict conclusions regarding long-term endocrine adaptation or translational applicability

for others, it may take up to 90 days to notice any visible results

His wins allowed his new school to eke out the team title by one point

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected