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Conditions like pernicious anaemia (an autoimmune condition affecting a protein called intrinsic factor vital for B12 absorption), stomach issues (such as from reduced stomach acid or certain medications like proton pump inhibitors), intestinal disorders (like Crohns disease or coeliac disease), or even surgical removal of parts of the small intestine (e.g., gastric bypass surgery) can prevent proper absorption

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The Arg-280 residue stably interacts with guanine bases in the major groove region of the target DNA, whereas the interactions involving Lys-120, Cys-277, and Ala-276 are sequence specific

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