Rajauria, S., Axline, C., Gottstein, C
The RAD51 fraction was further purified on a 1 ml HiTrap Heparin HP column (Cytiva, Cat#17040701) with a gradient of Buffer B ranging from 150 to 900 mM KCl
The fluorescence reading was performed on the GloMax Explorer plate reader (Promega) using excitation and emission filters of 520 nm and 580 to 640 nm (green), respectively
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