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Diabetes 56 , 24202424 (2007)

51 SongX.WangL.LiX.ChenZ.LiangG.LengX

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GHt Holz, Kuhtreiber W.M., Habener J.F

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After incubating for 30 min, excess metal ions were removed by buffer exchanging three times with buffer A, followed by two exchanges with 100 mM sodium phosphate buffer (pH 7.4) containing 1 mM cysteine