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Plant Physiol 155:218 Foyer CH, Rowell J, Walker D (1983) Measurements of the ascorbate content of spinach leaf protoplasts and chloroplasts during illumination

Reaction mixture II for each sample was collected as follows: 1160 l of 0.1-M Tris-HCl buffer with 0.34-mM EDTA (pH 8.5) were sequentially added to 108 l of each supernatant containing residual GSH, and then at the same intervals, 9 l of a solution of 5,5-dithiobis(2-nitrobenzoic acid) or Ellmans reagent (4 mg/ml of absolute methanol) was added to each sample (in the same sequence of samples)

Front Plant Sci 5:566 Zeid FA, Shihy OME, Ghallab AERM, Ibrahim FEZA (2009) Effect of exogenous ascorbic acid on wheat tolerance to salinity stress conditions

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doi: 10.1016/j.chemosphere.2008.11.023