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Generation of NEDD8-conjugated CUL1-RBX1 CUL1 neddylation was performed by incubating 8 M CUL1-RBX1, 500 nM APPBP1-UBA3, 1 M UBC12, 10 M NEDD8, and neddylation buffer (50 mM Tris pH 7.6, 100 mM NaCl, 2.5 mM MgCl 2 , 150 uM ATP) at room temperature for 10 min

doi:10.1152/AJPENDO.2001.280.5.E761 3

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Glutathione is a super antioxidant involved in the cancer protective properties in every cell within the human body