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NONSTEROIDAL ANTI-INFLAMMATORY DRUGS MECHANISM OF ACTION The nonsteroidal anti-inflammatory drugs comprise a large family of weak acidic drugs whose pharmacological effects result primarily from the inhibition of cyclooxygenase (COX) , an enzyme that catalyzes the first step in the synthesis of prostaglandins from arachidonic acid and other precursor fatty acids (see Chapter 26)

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LY294002 and SP600125 were from EMD (Gibbstown, NJ, USA) Western blot analysis The antibodies used for western blotting included those against stress-activated protein kinase (SAPK)/JNK, phospho-SAPK/JNK (Thr183/Tyr185), caspase-7, caspase-9, phospho-Bcl-2 (Ser70), and poly(ADP-ribose) polymerase (PARP) (Cell Signaling Technology, Danvers, MA, USA), cytochrome c and -actin (Sigma, St Louis, MO, USA), cytochrome oxidase subunit IV (Cox IV

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18 19 Issues with Nrf2 activation Whilst Nrf2 activation is undoubtedly beneficial in the vast majority of cases, there are some instances where it may prove harmful