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In contrast, metformin use was high in both groups (90% vs 71%, p = 0.19)

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To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit

When we talk about losing fat for health, the type of fat that often matters most is visceral fat the fat that accumulates deep in your abdomen around your internal organs (liver, pancreas, intestines, etc.)

Borghi 2006)

Translational Vision Science & Technology, 10 (8), 4