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Ligand-mediated intracellular calcium mobilisation was determined using a FlexStation (Molecular Devices, USA), with excitation wavelength at 485 nm and emission wavelength at 525 nm every 1.5 sec for 2 minutes, including a 15-second baseline with addition of agonists at increasing concentrations

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These deficits also trigger a surge in ghrelin, the hormone that makes you feel ravenously hungry

Nat Rev Endocrinol 11 , 289297 (2015)

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Many patients with Hashimoto's successfully use these treatments