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10.1177/0706743716635535 257 LeeB.KwonJ.-T.JeongY.CarisH.OhD.FengM.et al

Pyruvate kinase (encoding by pykF ) catalyzes phosphoenolpyruvic acid (PEP) to form pyruvate and is a rate-limiting enzyme in the glycolysis pathway

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For quantitative immunohistochemistry, the relative protein expression content/area determined from 160170 fields (40) of pancreas from each rat was quantified using ImageJ software