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2025;20(1):e0317202

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The following antibodies were used for ChIP assay: anti-ACTL6A (Abcam, ab3882), anti-NRF2 (Cell Signaling Technology, 12721 s), and Rabbit IgG (ab37415) (Abcam, Cambridge, MA)

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Regarding the first primary endpoint of change in heart failure-related symptoms and physical limitations from baseline to 52 weeks, semaglutide improved KCCQ-CSS in all diuretic subgroups, but the magnitude of improvement was greater in patients receiving loop diuretics compared to those not on loop diuretics, with an adjusted mean difference vs

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