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In hepatocyte cultures, retatrutide produces a complex pattern reflecting the competing effects of glucagon receptor activation (raising glucose output and lipid oxidation) and GLP-1 signalling (lowering glucose output and lipogenesis)

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Rapid injection forces medication into the tissue faster than it can accommodate, creating a pressurized pocket that causes pain and can push medication back out through the needle track

10.1371/journal.pone.0181880 12 BnemannM.GerhardsteinB

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