Membranes were pretreated with 5% non-fat milk/TBST (1 h) before overnight exposure at 4 C to antibodies targeting MAPKAPK2 (ab32567), HO-1 (ab189491), KEAP1 (ab119403), Nrf2 (ab137550), -H2AX (ab81299), PARP-1 (ab191217), CHK2 (ab207446), phosphorylated CHK2 (p-CHK2, ab59408), TYR (ab170905), PMEL (ab137078), MITF (ab12039), p-HSP27 (ab155987), HSP27 (ab12351), -actin (ab8226), and Histone H3 (ab1791) (all from Abcam, UK)
Each batch undergoes rigorous quality control, including identity and purity verification, and Certificates of Analysis (CoA) can be provided on request
Peaks weeks 3-4
For a deeper look at that philosophy, our Supplement Guide is a helpful next step
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Huang S, Zhang Y, Wang L, et al