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Cell counting kit (CCK)-8 analysis KGN cells (2 103 cells/well, 100 L/well) were plated in 96-well plates and cultured overnight, followed by continued culture for 72 h for CCK-8 assay

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Male C57BL/6N mice were fed a 60% fat diet from 9 weeks of age until they became obese (body weight 40 g at 15-21-week old

Losing muscle mass lowers metabolism and makes it harder to keep the weight off long term." Beth Samuelson, Registered Dietitian Nutritionist, Lakes Regional Healthcare Another concern

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