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Add in the potential for an itchy, inflamed scalp, a greasy finish, and the reality of long-term dependency to maintain results, and its easy to see why some people abandon the treatment before it starts working

10.1016/j.tim.2015.02.005 Trends Microbiol

Step 3: Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37C

To make it short it's one heck of an anti-oxidant in combating those free radicals