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Importantly, the sequence of testing did not affect the magnitude of 2MT-induced analgesia (Supplementary Fig

Ethics statement The animal study was reviewed and approved by Hebei General Hospital institutional animal care and conducted according to the AAALAC and the IACUC guidelines

Collectively, these untargeted metabolomic analyses indicate subtle but biologically relevant metabolic differences between ASD and control groups, characterized by increased variability and pathway-level alterations rather than complete global separation

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