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The protein extracts were separated by 12% SDS-polyacrylamide gel electrophoresis and then electroblotted onto polyvinylidene difluoride membranes

doi: 10.1016/bs.vh.2021.12.005 61 LarsenPJTang-ChristensenMHolstJJOrskovC

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Depending on the person, the dose, and the duration between doses, that practice can result in an individual having side effects when they do take their next dose, she says