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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

Inspiratory motoneuron activity was monitored through recordings from the ventral root of the fourth cervical spinal segment (C4)

Validation of ICD-9 codes with a high positive predictive value for incident strokes resulting in hospitalization using Medicaid health data

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Edwards LJ, Sharrack B, Ismail A, Tench CR, Gran B, Dhungana S, Brettschneider J, Tumani H

Bibcode:1991BBRC..179...78H