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Cells were transfected with siEPAC1 or siEPAC2 and pooled siRNAs at 0.1 nM concentration each using Lipofectamine RNAiMax transfection reagent according to manufacturers instructions

Metformin-induced apoptosis facilitates degradation of the cellular caspase 8 (FLICE)-like inhibitory protein through a caspase-dependent pathway in human renal cell carcinoma A498 cells

Front Pharmacol (2020) 11:1061

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While multiple studies suggest possible roles across diverse pathways, results remain variable and frequently preliminary, underscoring the need for further controlled investigations