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The crude material was purified by Isolera flash column chromatography on silica via gradient elution, from 100% dichloromethane to 15% methanol in dichloromethane to produce CAM-S-CAM-TPP (9.1 mg, 16%) as a pale yellow fluffy/foamy solid

It discusses the role of inflammatory cytokines, reactive oxygen species (ROS) generated from copper (cuproptosis) or iron (ferroptosis), and immunological observations in selected cases that may be classified as copper-induced autoimmune hepatitis (CIAIH)

doi: 10.1126/sciadv.aaw4543

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