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Preparation Condition 2 (EDTA deproteinization 80 o C): Heparinization and EDTA-treatment of plasma result in different levels of free GSH, GSSG, and their relative ratio, but does not affect stability of total GSH Suggesting that total GSH is a valid measure in both heparin and EDTA-treated plasma, we found no difference in total GSH between the heparinized plasma (Preparation Condition 1) and EDTA-treated plasma (Preparation Condition 2) stored at 80 o C (Fig

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Cancer Epidemiol Prev

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2017;26(3):177-81 Weschawalit S, Thongthip S, Phutrakool P, Asawanonda P

First, while the rescue experiments with ferric citrate and NAC strongly support an iron deficiency and ROS-mediated mechanism, the absence of established positive controls, such as the iron chelator deferoxamine (DFO) or direct ROS generators, precludes a definitive comparative conclusion that the observed effects are uniquely attributable to CGAs iron-chelating activity