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For samples frozen on holey carbon grids, the Vitrobot was maintained at 4LC with 100% humidity, using blotting times of 3.5Ls to 4.5Ls, blotting force of 0, and a waiting time of 10Ls

Townsend RR, Machin I, Ren J, Trujillo A, Kawaguchi M, Vijapurkar U, Damaraju CV, Pfeifer M

Glutathione is also an important storage and transport form of reduced sulfur owing to the thiol-containing Cys residue incorporated into the tripeptide and its biosynthesis is strongly dependent on the sulfur assimilatory pathway including necessary feedback inhibition mechanisms 63

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Post hoc sample size calculations were performed to guide recruitment efforts