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Liu Y-Y, Huo D, Zeng L-T, Fan G-Q, Shen T, Zhang T-M, Cai J-P, Cui J

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A 25 l of mouse plasma was transferred into 1.5 ml centrifuge tube which was spiked with 2.5 l of internal standard (IS) working standard solution (100 g/ml) to get final IS contraction of 250 ng/ml and the solution was vortexed