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Thus, treatment of LNCaP cells with the DNA demethylating agent 5-azacytidine also restored activating histone modifications on GSTP1 and as a result reactivated transcription

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References: [1]Potapenko, Dmitrii I

To the 10.000 rpm supernatant containing microsomes and cytosol fractions, 0.2 volume of 0.1 M calcium chloride solution was added, and further centrifuged at 10,000 rpm for 10 minutes to precipitate the microsomes

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