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Thiamine deficiency adversely affects the activity levels of these enzymes, with TK, through thiamine-dependent erythrocyte transketolase (ETK) activity, common biomarkers of thiamine status, since it is particularly sensitive to thiamine levels and AKD often exhibiting the earliest detectable changes (41)

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The same study also revealed that ergosterol levels in the cell membranes of biofilm-growing cells decreased with biofilm age compared to planktonic cells, which could lead to reduced efficacy of ergosterol-targeting drugs, such as nystatin, on more mature biofilms