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Cells were washed once in 1.25 M glycine/PBS and lysed for 60 min on ice with homogenization in 1 mL RIPA buffer (50 mM Tris HCl, pH 8.0, 150 mM sodium chloride, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, protease inhibitors (Complete mini, EDTA-free, Roche))

[33] Retinoic acid decreased the growth rate and increased differentiation of human small cell lung cancer lines in vitro

10.1258/002367787781268693 Lab Anim

Insulin signalling and the regulation of glucose and lipid metabolism

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