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The concentration of mRNA was determined using NanoDrop 1000 Spectrophotometer (Thermo Scientific, USA)

Mitchell KG, Bayley EM, Ikoma N, Antonoff MB, Mehran RJ, Rajaram R, Rice DC, Roth JA, Sepesi B, Swisher SG , Vaporciyan AA, Walsh GL, Maru DM, Erasmus JJ, Weston BR, Ajani JA, Badgwell BD, Hofstetter WL

As hTERT codes for the catalytic subunit of the functional telomerase enzyme, telomerase activity was quantified using qPCR to determine if epitalon enhances its activity through hTERT expression

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N.LaxminarayanR.PerencevichE

In database search, full trypsin digestion specificity was required, and up to two missed cleavages were allowed