Iron-regulated expression of a cytosolic ascorbate peroxidase encoded by the APX1 gene in Arabidopsis seedlings
Cell viability was measured using the CellTiterBlue reagent (Promega, United States) according to the manufacturers protocol with Fluoroskan Ascent FL Microplate Reader (Thermo Labsystems, Waltham, MA, United States)
Glutathione represents the most abundant intracellular antioxidant capable of (i) directly interacting with free radical species, (ii) revitalizing other small molecule antioxidants (e.g., vitamins C or E), (iii) maintaining the redox state of protein thiols, and (iv) serving as a cofactor for enzymes of the cellular antioxidant system (Espinosa-Diez et al
This interconnected network of antioxidants helps sustain the bodys defense against oxidative damage, reinforcing its importance in maintaining health
Scientists have extensively studied the health benefits of glutathione, linking it to everything from improved liver function[7] and immune support[10] to clearer skin[13] and enhanced energy levels[5]
doi: 10.1002/jps.23325 227 RaftosJ