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Individuals experiencing symptoms such as fatigue, weakness, memory problems, or those with dietary restrictions (e.g., vegetarians and vegans) should consider B12 testing

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PCR products were subcloned (BP reaction, ThermoFisher Scientific) into pDONR/Zeo (ThermoFisher Scientific) before Gateway cloning into the pLX304 lentiviral expression vector (LR reaction, ThermoFisher Scientific)

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