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Upon presentation, additional laboratory studies are necessary to obtain other important clinical parameters, including arterial blood gas (to investigate acid/base status), coagulation profile, basic metabolic panel, hepatic function tests, and urine drug screen (to determine possible co-ingestions)

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Coronal sections (250 M thick) from a hypothalamic block were cut with a Leica VT1000S vibratome and then incubated in oxygenated aCSF at room temperature for at least 1 h before recording

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