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For scRNA-seq library preparation, cells were loaded onto the 10 Genomics Chromium instrument and 10 Genomics Chromium Single-Cell 3kit V3 reagents were used according to the manufacturers protocols

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Huang J, Agus DB, Winfree CJ, Kiss S, Mack WJ, McTaggart RA, Choudhri TF, Kim LJ, Mocco J, Pinsky DJ, Fox WD, Israel RJ, Boyd TA, Golde DW, Connolly ES: Dehydroascorbic acid, a blood-brain barrier transportable form of vitamin C, mediates potent cerebroprotection in experimental stroke

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