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Methods Construct design We initially tried expression of the full-length rat GluK3 subunit containing point mutants R591Q (Q/R site) but this construct had very low expression and poor profile on size exclusion chromatography (SEC) and hence was not suitable for structural analysis

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During the procedure, a small puncture is made in an artery, typically in the groin or wrist, to insert a microcathetera tiny, flexible tubeinto the prostate arteries