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For analyses of GLP-1, glucagon, and Ex9-39, blood was sampled into chilled tubes containing EDTA and a specific DPP-4 inhibitor (3 mmol/l valine-pyrrolidide, 10 l/ml blood, a gift from Novo Nordisk) and kept on ice until centrifugation

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The DPP4 inhibitor washout period of 56 days prior to randomization was included for participants treated with any DPP4 inhibitor at entry into the study in order to discontinue the DPP4 inhibitor treatment

Quality and relevance assessment Study relevance: Each source was evaluated for its relevance to the key themes of the review

Relative Scale (1 to 36): 1 = lower copper / 36 = highest copper

GLP-1 corresponde a glucagon-like peptide-1 , una hormona producida de manera natural por el organismo, cuya funcin principal es regular los niveles de azcar en la sangre y el apetito