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Some lactobacilli are folate auxotrophs dependent on folate intake from exogenous sources

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From the perspective of mitochondrial iron metabolism, iron-export transporters such as ferroportin 1 (FPN1) and ATP-binding cassette subfamily B member 8 (ABCB8) are crucial for maintaining mFe homeostasis

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Cells were lysed in 1.5 mL of RIPA buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) 100 for 30 min at 4 C