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However, levels of glutathione decrease due to factors like aging, UV exposure, and poor lifestyle habits

Studies indicate that glutathione supplementation influences skin pigmentation, protects against UV-induced damage, and enhances overall skin brightness through antioxidant mechanisms [72, 74]

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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