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For age-related effects, we calculated the average age of the healthy control (HC) group and identified metabolites below the average age (BAA) or above the average age (UAA) threshold

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The extraction from a sample of 100 l whole blood was done in non-buffered mixture of alcohols heated to induce protein unfolding 27 , releasing non-covalently bound cofactors while preserving redox-sensitive metabolites

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