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We found that blocking the translation of NRF2 prevented GSH accumulation ( Figure 7F ) as well as negated the immunosuppressive action of melatonin treatment ( Figures 7H, I )

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2.13 Mass spectrometry 2.13.1 Hydrogen-deuterium exchange mass spectrometry (HDX-MS) Time-resolved spontaneous hydrogen-deuterium exchange (HDX) of covalently bonded hydrogens of a protein with deuterium in solution provides information about protein conformation, flexibility, and solvent accessibility (Englander et al., 2016)

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Step 5: The Gentle Mix Once all the diluent is in the vial, remove the syringe