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Metabolomic analysis We used Biocrates AbsoluteIDQ p180 kit to analyze cardiac metabolites from female mice [Young ( n = 7), Old Con ( n = 8), and Old mAb ( n = 7)] with UPLC-MS/MS Xevo TQ, Waters, Pittsburgh, PA, USA) in the Einstein Stable Isotope and Metabolomics Core, according to the manufacturers instructions (BIOCRATES Life Sciences AG, Innsbruck, Austria)

*Hormonal birth control was defined using claims for oral contraceptives, the patch, vaginal ring, implant, hormonal intrauterine device, and injectable medroxyprogesterone acetate in the two years before diagnosis

Due to all these factors, supplementation of L-Carnitine becomes essential for fat loss

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