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STAT3 activation in HER2-overexpressing breast cancer promotes epithelial-mesenchymal transition and cancer stem cell traits

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For each z-slice, a relative signal was obtained by calculating the ratio intensity (mean gray value) for Ubx signal (488 nm) relative to the DAPI signal in T2-T3 segments (which only expressed ectopic Ubx protein) using the same region of interest (ROI)