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Analytical size-exclusion chromatography (SEC) In order to analyze the ability of SnCE1 and variants thereof to form higher oligomers, analytical SEC was performed using a calibrated Superdex 200 10/300 GL column (Cytiva Germany GmbH, Freiburg DE) (Supplementary Figs

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This hypothesis is in accordance with a previous observation [59], which reported that amino acids linked to tricarboxylic acid cycle, such glutamate and aspartate, undergo specific enrichment when young rats are fed with a high-fructose diet